Vol. 30, Issue 2-3, pp. 231-238

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  2. Optica Applicata Vol. 30, Issue 2-3, pp. 231-238 (2000)
Vol. 30 Issue 2-3 pp. 231-238

Nonlinear delayed fluorescence in confocal scanning microscopy. Point-spread-function analysis

Kowalczyk Marek, Martinez-Corral Manuel, Rodriguez Carlos J.Z.

Abstract

Generation of the signal beam in the confocal scanning fluorescence microscopy by means of nonlinear delayed fluorescence phenomenon based on triplet-triplet annihilation is proposed. From the point of view of the spatial resolution of three-dimensional confocal imaging the superiority of the phenomenon proposed over the classical two-photon fluorescence is demonstrated. This is done in terms of axial and lateral point spread functions computed for the assumed model of nonlinearity.

Vol. 30
Issue 2-3
pp. 231-238

0.39 MB
OPTICA APPLICATA - a quarterly of the Wrocław University of Science and Technology, Faculty of Fundamental Problems of Technology