Vol. 30, Issue 2-3, pp. 455-461

Vol. 30 Issue 2-3 pp. 455-461

Point spread function in a confocal microscope with trigonometric pupil filters

Magiera Anna

Abstract

The distribution of point spread function was examined versus the spatial frequencies of the filters of cos(Np) type modulating the aperture of the confocal CSM for different values of numerical aperture. In particular, the following relations were determined: (i) PSF as dependent on the pupil modulating spatial frequency r for N=1, 2, 3, 4, 5, 6, 7, 9, 11 and numerical aperture NA=0.8; (ii) PSF for filters of cos(4ρ), cos(10ρ) type and NA=0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4; (iii) cut-off spatial frequencies rc for the aperture modulated by cos(Nρ) for N=0,..., 20, NA=0.2, 0.5, 1.0 and λ =0.6328 μm.

Vol. 30
Issue 2-3
pp. 455-461

0.45 MB
OPTICA APPLICATA - a quarterly of the Wrocław University of Science and Technology, Faculty of Fundamental Problems of Technology