Vol. 33, Issue 2-3, pp. 369-380

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  2. Optica Applicata Vol. 33, Issue 2-3, pp. 369-380 (2003)
Vol. 33 Issue 2-3 pp. 369-380

Application of principal component and factor analysis of fluorescence spectra in camptothecin studies

Stefan Kruszewski, Ryszard Siuda, Blanka Ziomkowska, Michal Cyrankiewicz

Keywords

camptothecin, fluorescence, principal component analysis, factor analysis

Abstract

The application of fluorescence spectroscopy methods in investigations of camptothecin (CPT) is presented in this paper. Fluorescence of CPT enables one to follow the process of hydrolysis, i.e., the process of converting the biologically active lactone form into inactive carboxylate. The fluorescence spectra of CPT recorded during the hydrolysis were analysed using principal component analysis (PCA) and factor analysis (FA). The results obtained on the basis of fluorescence spectra analysis are compared with high performance liquid chromatography (HPLC) data.

Vol. 33
Issue 2-3
pp. 369-380

0.68 MB
OPTICA APPLICATA - a quarterly of the Wrocław University of Science and Technology, Faculty of Fundamental Problems of Technology