Vol. 28, Issue 2, pp. 127-138

Vol. 28 Issue 2 pp. 127-138

Phase pupil filters for improvement of the axial resolution in confocal scanning microscopy

Marek Kowalczyk, Carlos Zapata, Enrique Silvestre, Manuel Martinez-Corral

Abstract

Two phase pupil filters whose axial impulse response is asymmetric with respect to the focal point are proposed. It is shown that when either of the filters is placed both in the illuminating and collecting part of the system the impulse response becomes symmetric and the axial resolution increases. The filters are designed on the basis of the Fourier-transform properties of the Hermitian functions. They consist of a small number of zones (< 3) whose amplitude transmittance is constant and its module is equal to unity. The width of all zones is adequately chosen. Several quantitative characteristics of the proposed filters which evidence the improvement of the axial resolution are presented.

Vol. 28
Issue 2
pp. 127-138

2.77 MB
OPTICA APPLICATA - a quarterly of the Wrocław University of Science and Technology, Faculty of Fundamental Problems of Technology