Vol. 30, Issue 2-3, pp. 231-238 (2000)

Abstract

Generation of the signal beam in the confocal scanning fluorescence microscopy by means of nonlinear delayed fluorescence phenomenon based on triplet-triplet annihilation is proposed. From the point of view of the spatial resolution of three-dimensional confocal imaging the superiority of the phenomenon proposed over the classical two-photon fluorescence is demonstrated. This is done in terms of axial and lateral point spread functions computed for the assumed model of nonlinearity.