Vol. 33, Issue 2-3, pp. 369-380 (2003)

Abstract

The application of fluorescence spectroscopy methods in investigations of camptothecin (CPT) is presented in this paper. Fluorescence of CPT enables one to follow the process of hydrolysis, i.e., the process of converting the biologically active lactone form into inactive carboxylate. The fluorescence spectra of CPT recorded during the hydrolysis were analysed using principal component analysis (PCA) and factor analysis (FA). The results obtained on the basis of fluorescence spectra analysis are compared with high performance liquid chromatography (HPLC) data.