Vol. 41, Issue 2, pp. 395-401 (2011)

Abstract

Flow cytometry is a very popular clinical diagnostic method for a fast analysis of different kinds of cells/microparticles. In classic cytometers fluorescence, labeled cells are hydrodynamically focused in flow stream to order them and direct individually to a detection area with an optical detector. After laser excitation, the fluorescence emission light is directed into a light sensitive window of an optical detector (photomultiplier). Output signals of the detector may be counted in the digital or analog form (integration). In the experimental system, the photomultiplier was used as the integrator, so its output voltage was proportional to the number of fluorescence labeled cells. To miniaturize the flow cytometer, own technology for fabrication of microfluidic structure as a pre-concentrator with utilization of SU-8 masters was used.