Vol. 56, Issue 1, pp. 5-17 (2026)

Abstract

In the context of digital holographic microscopic quantitative phase imaging, a microscope is employed in the experimental setup to magnify both the object light and the reference light beam. The three-dimensional phase information within the hologram is influenced by the curvature of the microscope, resulting in significant distortion of the object phase. Additionally, off-axis tilting introduces further phase distortions. In this study, we propose a two-wavelength phase subtraction method to mitigate such distortions in digital holographic microscopy. The procedure involves capturing phase images at distinct wavelengths using filters that are highly transparent to the 671 nm band and highly reflective to the 532 nm band. By subtracting the phase image containing both object information and distortion from the phase image containing only distortion, a distortion-compensated phase image is obtained. Through both theoretical analysis and experimental validation, we demonstrate that the proposed method offers substantial potential for effectively compensating distortions in digital holographic microscopy.